Dictyostelium discoideum: a new method for cloning in liquid medium.
نویسنده
چکیده
Dictyostelium discoideum can be cloned in droplets of liquid medium by a method that permits observation of the complete life cycle. This alternative to solidified media provides advantages in clonal analysis.
منابع مشابه
Growth of myxameobae of the cellular slime mould Dictyostelium discoideum in axenic culture.
1. A simple axenic medium suitable for the growth of the myxamoebae of a strain of the cellular slime mould Dictyostelium discoideum is described. 2. Procedures suitable for the growth of this strain in liquid and on solid media are described. 3. Conditions suitable for initiating the cell differentiation of myxamoebae grown axenically are described.
متن کاملA defined minimal medium for axenic strains of Dictyostelium discoideum.
A defined, minimal medium for the cellular slime mold Dictyostelium discoideum is reported.
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Charles L. Rutherford, Pawjai Khampang, Xiao Wen, Reyna Favis and Ian McCffery. Purification and Identification of a Protein that Binds to Regulatory Sites of the Glycogen Phosphorylase-2 Promoter of Dictyosteliym discoideum . International Dictyostelium Conference, October 13-18, 1996, Sendai, Japan
متن کاملObservations on the growth of Dictyostelium discoideum in axenic medium: evidence for an extracellular growth inhibitor synthesized by stationary phase cells.
By analysing the growth kinetics of Dictyostelium discoideum amoebae in varying combinations of the components of the nutrient medium HL-5 (Cocucci & Sussman, 1970), we have demonstrated that Dictyostelium amoebae enter stationary phase when they deplete the medium of one or more nutrient components. However, if the medium of a stationary phase culture is supplemented with any of the initial co...
متن کاملSymbolical Kernel-Based Reasoning: Its Application to the Rule Extraction in Dictyostelium discoideum DNA
Kernel-based method is one of the promising algorithms in the pattern recognition field [1]. We have extended the algorithm to the symbolical reasoning tasks (we call the algorithm symbolical kernel-based reasoning (SKBR)) [2], and we currently apply the SKBR to the rule extraction in Dictyostelium discoideum the DNA and cDNA databases of which are well established already [3]. Goal of our proj...
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عنوان ژورنال:
- Journal of bacteriology
دوره 117 3 شماره
صفحات -
تاریخ انتشار 1974